ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Theoretical Study of Discrimination of Mechanisms of Thermal Inactivation of Urease by Monitoring its Oligomeric Composition

V. Illeová and M. Polakovič

Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-812 37 Bratislava

E-mail: viera.illeova@stuba.sk

Received: 1 April 2003

Abstract: The possibility to discriminate the three-step mechanisms of thermal inactivation of jack bean urease was analyzed, the models being suggested in a previous study from the activity data. The simulations of the concentration courses of individual structural forms of urease incorporated in the mechanisms were performed. The results showed that all mechanisms could be discriminated if the dynamics of the evolution of oligomeric structural forms of urease was monitored. A careful choice of temperatures and combination of several analytical techniques is, however, required to achieve a reliable discrimination of the inactivation mechanism.

Full paper in Portable Document Format: 581a64.pdf

Chemical Papers 58 (1) 64–70 (2004)