ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Selective chemisorbents. IV. Cleavable binding of thiols to S-alkylthiosulfate derivatives of cellulose

P. Gemeiner and L. Petruš

Institute of Chemistry, Centre for Chemical Research, Slovak Academy of Sciences, CS-842 38 Bratislava

Abstract:  It has been found that bead and powder 0-(3-thiosulfato-2-hydroxypropyl)celluloses adsorb low- and high-molecular-mass thiols. The efficiency of sorption of low-molecular-mass thiols was controlled by mass ratio of thiol and S₂O₃^-. In removing cysteine from aqueous medium it was necessary to use large excess of S-alkylthiosulfate groups of the sorbent. Chemisorption of papain was inhibited by side reactions. Desorption of papain and cysteine was accomplished by addition of thiols in weak alkali medium. The purity of papain increased after desorption. One of the sorbents, bead 0-(3-thiosulfato-2-hydroxypropyl)cellulose, was prepared by the reaction of bead 0-(3-iodo-2-hydroxypropyl)cellulose with sodium thiosulfate.

Full paper in Portable Document Format: 384a531.pdf

Chemical Papers 38 (4) 531–538 (1984)