ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Purification of phloridzin from Lithocarpus polystachyus Rehd leaves using polymeric adsorbents functionalized with glucosamine and β-cyclodextrin

Qian-Wen Wang, Jia-Jia Liu, Xiong Liu, Hong-Wei Zha, and Na Ren

College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, Hunan, China

E-mail: liujj0903@163.net

Abstract: A high-efficient purification method of flavonoids from Lithocarpus polystachyus Rehd leaves is reported. Two adsorbents functionalized with glucosamine (GA) and β-cyclodextrin (β-CD) were synthesized and characterized by the Fourier transform infrared (FTIR) spectra. Also, adsorption properties and purification effect of flavonoids on different adsorbents were investigated. Two typical commercial adsorbents, AB-8 and D101, were employed as the reference materials. The adsorption capacity was enhanced remarkably by the introduction of GA and β-CD groups to the adsorbents. Moreover, the isotherms were well fitted by the Freundlich model. After a one-step column chromatographic separation, the purity of phloridzin increased significantly from 14.65 % to 79.48 % for PS-CD, to 66.56 % for PS-GA, 44.68 % for D101, and to 52.84 % for AB-8. This study provides a novel alternative matrix for the purification of phloridzin from Lithocarpus polystachyus Rehd leaves extracts.

Keywords: macroporous adsorption resin – purification – phloridzin – hydrogen-bond

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-014-0615-x

Chemical Papers 68 (11) 1521–1531 (2014)