ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Validation of a colorimetric method for determination of paracetamol in exhaled breath condensate
Fariba Pourkarim, Elaheh Rahimpour, Maryam Khoubnasabjafari, Vahid Jouyban-Gharamaleki, Afshin Gharakhani, and Abolghasem Jouyban
Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Received: 19 September 2020 Accepted: 15 January 2021
A redox reaction-based colorimetric method was validated for the qualitative assay and quantitative determination of paracetamol in exhaled breath condensate (EBC). Fe (III) and K3[Fe(CN)6] were used as colorimetric reagents in which paracetamol reduces Fe (III) to Fe (II) due to its reducing property. The produced Fe (II) is chelated with K3[Fe(CN)6] to form prussian blue colored product which can be detected by the naked eye in the range of 0.2–10.0 μg mL−1. The fully validated method was used for the determination of paracetamol in EBC of paracetamol poisoning individuals.
Keywords: Paracetamol; Exhaled breath condensate; Redox reaction; Colorimetry
Full paper is available at www.springerlink.com.
Chemical Papers 75 (6) 2901–2906 (2021)