ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Validation of a colorimetric method for determination of paracetamol in exhaled breath condensate

Fariba Pourkarim, Elaheh Rahimpour, Maryam Khoubnasabjafari, Vahid Jouyban-Gharamaleki, Afshin Gharakhani, and Abolghasem Jouyban

Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

E-mail: ajouyban@hotmail.com

Received: 19 September 2020  Accepted: 15 January 2021

Abstract:

A redox reaction-based colorimetric method was validated for the qualitative assay and quantitative determination of paracetamol in exhaled breath condensate (EBC). Fe (III) and K₃[Fe(CN)₆] were used as colorimetric reagents in which paracetamol reduces Fe (III) to Fe (II) due to its reducing property. The produced Fe (II) is chelated with K₃[Fe(CN)₆] to form prussian blue colored product which can be detected by the naked eye in the range of 0.2–10.0 μg mL⁻¹. The fully validated method was used for the determination of paracetamol in EBC of paracetamol poisoning individuals.

Keywords: Paracetamol; Exhaled breath condensate; Redox reaction; Colorimetry

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-021-01520-2

Chemical Papers 75 (6) 2901–2906 (2021)