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Rapid synthesis of silver nanoparticles using the extract of Psidium guajava leaf based on light-emitting diodes irradiation

Trung Dien Nguyen, Dam Phuc Nguyen, Yen Hai Hoang, Tuan Trong Nguyen, and Hong Thi Nguyen

School of Education, Can Tho University, Can Tho, Vietnam



Received: 5 March 2021  Accepted: 15 June 2021


A green synthesis of silver nanoparticles (AgNPs) was conducted by using Psidium guajava (P. guajava) leaf extract under light-emitting diodes (LED) irradiation. The obtained AgNPs were characterized by ultraviolet–visible spectroscopy, X-ray diffraction , energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and transmission electron microscopy (TEM). In comparison to the absence of LED irradiation, the rate of AgNPs formation has been increased by more than twice with LED irradiation. Among LED irradiations, blue LED offered the most efficient in the formation of AgNPs. TEM analysis revealed that both AgNPs-N (without LED irradiation) and AgNPs-L (with LED irradiation) were nearly spherical with an average size of 32 nm.The antimicrobial assay indicated that AgNPs-L exhibited better antimicrobateria than AgNPs-N. The best antibacterial activity of AgNPs-L was recognized on L. fermentum (IC50 = 4.0 pM) for gram-negative bacteria, P. aeruginosa (IC50 = 3.5 pM) for gram-positive bacteria, and C. albicans (IC50 = 4.6 pM) for fungi.

Keywords: Silver nanoparticles; Antibacterial activity; Green synthesis; LED irradiation; P. guajava

Full paper is available at

DOI: 10.1007/s11696-021-01747-z


Chemical Papers 75 (11) 5623–5631 (2021)

Tuesday, May 28, 2024

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