ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

Deila Magna dos Santos Botelho, Mário Lúcio Vilela de Resende, Alexandre Rezende Teixeira, Wilder Douglas Santiago, Edson Ampélio Pozza, Silvino Intra Moreira, Juliana Barros Ramos, Sinara Oliveira de Aquino, Antônio Gilberto Ferreira, Tharyn Reichel, and Leonor Guerra-Guimarães

Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Brazil

E-mail: deilamagna@hotmail.com

Received: 27 June 2021  Accepted: 2 November 2021

Abstract:

Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production.

Keywords: Microscopy; Toxin; High-performance liquid chromatography; Methods

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-021-01965-5

Chemical Papers 76 (4) 2567–2572 (2022)