ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Aptamer labeled nanozyme-based ELISA for ampicillin residue detection in milk

Xuping Zhang, Wenxia Sun, and Yuan Cheng

Chengdu Second People’s Hospital, Chengdu, China

E-mail: chengyuan83@cdutcm.edu.cn

Received: 3 September 2021  Accepted: 13 January 2022

Abstract:

Abstract

This study aimed to develop an aptamer labeled nanozyme-based ELISA method based on ampicillin/aptamer and peroxidase activity of gold nanoparticles (AuNPs) to detect ampicillin residue in milk. The commercially available ampicillin conjugated bovine serum albumin (ampicillin/BSA) was used as a ‘coating antigen.’ The aptamers labeled with AuNPs (Au-apt) were prepared and used as an ‘enzyme-labeled antibody’ to detect ampicillin in milk. A positive correlation was observed between the amounts of ampicillin residue and competitively bounded Au-apt. The remaining Au-apt bounded ampicillin/BSA was negatively associated with UV absorption values. The materials are easy to be prepared in the present method. This study simplified the material preparation and demonstrated high a selectivity, a low detection limit, and a good batch stability. The method developed in this study has the potential to improve the monitoring method of ampicillin residue in milk.

Graphical abstract

Keywords: Ampicillin residue; Milk; Aptamer; Nanozyme

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02084-5

Chemical Papers 76 (5) 3077–3085 (2022)