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Green synthesis of highly pure copper nanoparticles under microwave irradiation against pathogenic fungi on plants

Trung Dien Nguyen, Hong Thi Nguyen, Nghia Khoi Nguyen, Hang Thi Phung, and Yen Hai Hoang

School of Education, Can Tho University, Can Tho, Vietnam



Received: 27 December 2021  Accepted: 2 March 2022


A rapid synthesis process of copper nanoparticles (CuNPs) using green agents was investigated in the present work. In the synthesis process of CuNPs, L-ascorbic acid and starch were used as reducing and stabilizing agents, respectively, while the presence of microwave irradiation enhanced the formation rate of CuNPs. A starch concentration of 10 g/L, 20 mM Cu(AcO)2, 80 mM L-ascorbic acid, the power level of 360 W, and reaction time of 120 s were found as optimal conditions for CuNPs formation. Under the optimum condition, the synthesis efficiency of CuNPs was approximately 89.7%. The synthesized CuNPs possessed an absorption peak at a maximum wavelength of 602 nm and spherical shapes with an average size of 13.6 nm. The quasi-sphere CuNPs were produced with a pure phase. Additionally, the synthesized CuNPs heightened antifungal activity on plants including Colletotrichum gloeosporioides (C. gloeosporioides) and Fusarium solani (F. solani). The antifungal activity of CuNPs against F. solani was better than that of C. gloeosporioides. The best inhibitory concentration for F. solani was 1.0 mM while the value of 1.5 mM CuNPs was found to be the best value for C. gloeosporioides inhibition. The obtained results indicated that the CuNPs are effective nanoparticles against fungal infection.

Keywords: Antifungal activity; Copper nanoparticles; Green synthesis; Microwave irradiation; Pure phase

Full paper is available at

DOI: 10.1007/s11696-022-02162-8


Chemical Papers 76 (7) 4159–4167 (2022)

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