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Development of epitope-imprinted polydopamine magnetic nanoparticles for selective recognition of allergenic egg ovalbumin

Tabkrich Khumsap, Suwussa Bamrungsap, Vu Thi Thu, and Loc T. Nguyen

School of Environment, Resources and Development, Asian Institute of Technology, Klong Luang, Thailand

 

E-mail: locnguyen@ait.ac.th

Received: 17 March 2022  Accepted: 15 May 2022

Abstract:

In this study, epitope-imprinted polydopamine (PDA) magnetic nanoparticles (EIP-MNP) were developed for selective separation of allergenic ovalbumin (OVA). The imprinting process was conducted through the polymerization of dopamine in the presence of OVA IgE-binding epitope as the template. Molecularly imprinted PDA was self-assembled on the surface of silica-functionalized magnetic nanoparticles (MNP@SiO2). Key parameters including template concentration, pH of precursor solution, and rebinding time were examined to optimize the recognition capability of EIP-MNPs. The results suggested that EIP-MNPs had high affinity to OVA with an imprinting factor of 7.52. The developed EIP-MNPs also exhibited good selectivity against bovine serum albumin, human serum albumin, and lysozyme. The recovery percentage of OVA from wine samples ranged from 95.4 to 105.7%, with the RSD from 3.32 to 5.69%. The method can be easily modified to separate other allergenic proteins from foods.

Graphical abstract

Keywords: Epitope imprinting; Polydopamine; Ovalbumin; Magnetic nanoparticles; Separation

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02291-0

 

Chemical Papers 76 (10) 6129–6139 (2022)

Wednesday, April 17, 2024

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