ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly

A new pyrene-based “turn-on” fluorescent probe for highly selective detection of hypochlorite in aqueous solution and in living cells

Nan Jiang, Xue Zhou, Tianyuan Zhong, and Guang Wang

Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Faculty of Chemistry, Northeast Normal University, Changchun, China



Received: 19 June 2022  Accepted: 4 September 2022


A new pyrene-based fluorescent probe (PM) for detection of hypochlorite was synthesized through the reaction of aldehyde with methylene and well characterized. PM presents highly selective fluorescence enhancement response toward OCl over a variety of different analytes, including metal ions, anions and some compounds with redox properties via the unique reaction of Meldrum’s acid with OCl. The results of mass spectra, FTIR spectra and density functional theory calculation demonstrated that the fluorescence enhancement of PM is attributed to the inhibition of intramolecular charge transfer due to the protonation of O atoms on Meldrum’s acid molecule after the oxidation reaction of OCl on PM. The fluorescence intensity of PM at 517 nm in aqueous solution displayed a good linear relationship with the concentration of OCl in a wide range with a low detection limit of 4.24 μM. Additionally, the viability of HepG-2 cell after being incubated in 15 μM PM solution for 24 h can reach 90%, indicating that PM has very low cytotoxicity. PM can recognize OCl in living cells by the fluorescent confocal imaging. PM has the potential application in detection and recognition of OCl in environmental systems and living organisms.

Graphical abstract

Keywords: Fluorescence probe; Turn-on; Hypochlorite; Intramolecular charge transfer; Bioimaging

Full paper is available at

DOI: 10.1007/s11696-022-02475-8


Chemical Papers 77 (1) 197–205 (2023)

Sunday, April 14, 2024

SCImago Journal Rank 2021
European Symposium on Analytical Spectrometry ESAS 2022
© 2024 Chemical Papers