ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Anticancer effects of Curcuma zedoaria (Berg.) Roscoe ethanol extract on a human breast cancer cell line

Nur Fitriana, Muhaimin Rifa’i, Masruri, Septian Tri Wicaksono, and Nashi Widodo

Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia

E-mail: widodo@ub.ac.id

Received: 10 May 2022  Accepted: 8 September 2022

Abstract:

Breast cancer is a malignant cancer with annually increasing incidence and mortality rates. The anticancer effect of Curcuma zedoaria (Berg.) Roscoe ethanol extract (ECz) on T47D breast cancer cells was investigated in vitro. The ECz contains phenolic and flavonoid compounds that may play a critical role in anticancer activity. The viability of the treated T47D cells was significantly reduced compared to that of the control after treatment with ECz. The extract was able to induce apoptosis, upregulate B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax), caspase-9, and caspase-3 protein expression levels, followed by a decrease in Bcl-2 levels and loss of mitochondrial membrane potential. In addition, the ECz also inhibited colony formation and cell migration. Signaling pathway analysis confirmed that ECz decreased the phosphorylated p38 mitogen-activated protein kinase levels. Taken together, the data suggest that ECz might be beneficial as a therapeutic agent for breast cancer.

Keywords: Breast cancer; Cell colony; Cell migration; C. zedoaria; Protein expression

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02482-9

Chemical Papers 77 (1) 399–411 (2023)