ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7

Published monthly

Carboxymethyl cellulose-NiO nanoparticles as peroxidase mimic for sensitive colorimetric detection of hydrogen peroxide

Shehala, Kirti Baranwal, Mani Prabha, Tulika Malviya, Anand Gaurav, and Vandana Singh

Department of Chemistry, University of Allahabad, Prayagraj, India



Received: 17 May 2022  Accepted: 25 July 2022


A simple co-precipitation method has been used to derive an excellent peroxidase mimetic material, carboxymethyl cellulose-stabilized NiO nanoparticles (Cm-NiONps). CMC provided uniqueness to Cm-NiONps by capping the incipient NiONps as the capping prevented any further agglomeration. Characterization study revealed that the nanoparticles are spherical to oblong in shape (~ 6 nm size), crystalline in nature, and possess coated morphology. Cm-NiONps exhibited excellent peroxidase mimetic behavior toward H2O2 oxidation of 3,3’,5,5’ tetramethyl benzidine (TMB) to produce a quick visual response (blue color) within an extremely short time span of 1.5 min. Cm-NiOcNps-catalyzed oxidation of TMB followed Michaelis–Menten behavior toward both H2O2 and TMB and indicated its very high affinity for both H2O2 (Km = 0.04/mM) and TMB (Km = 0.194/mM). Vmax for the enzymatic reaction is 12.67 × 10–8 M s−1 (wrt TMB) which is almost at par with the reported value for HRP. This colorimetric platform exhibited excellent sensitivity (LOD =  0.078 μM), reproducibility, low-temperature activity (at 35 °C), and high selectivity. The catalyst showed high recyclability as it conserved most of its activity even after being used in six consecutive cycles. The colorimetric method could successfully detect the presence of H2O2 levels in milk samples from local market and human serum.

Keywords: Carboxymethyl cellulose; NiO; Nanoparticles; H2O2; Sensing; TMB

Full paper is available at

DOI: 10.1007/s11696-022-02401-y


Chemical Papers 77 (3) 1299–1316 (2023)

Saturday, May 25, 2024

SCImago Journal Rank 2021
European Symposium on Analytical Spectrometry ESAS 2022
© 2024 Chemical Papers