ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Enhanced healing of skin wounds in ischemic rabbits using chitosan/hyaluronan/edaravone composite membranes: effects of laponite, carbon and silver-plated carbon nanofiber fillers

Katarína Valachová, Karol Švík, Rastislav Jurčík, Ľubomír Ondruška, Csaba Biró, and Ladislav Šoltés

Centre of Experimental Medicine, Bratislava, Slovakia

E-mail: katarina.valachova@savba.sk

Received: 20 July 2022  Accepted: 18 October 2022

Abstract:

We examined the free-radical scavenging capacity of edaravone using the standard spectroscopic methods. Further, the impact of wound dressings composed of chitosan/hyaluronan with the addition of H atom and/or electron donoring edaravone in the absence and presence of laponite, C-nanofibers or silver-plated C-nanofibers was assessed on the healing of skin lacerations in ischemic ears of rabbits. The skin wound healing was compared with the untreated animals and animals, in which the wound was protected by a bicomponent chitosan/hyaluronan membranes. The in vitro results showed that edaravone in a dose-dependent manner was both free electron and H atom donor. The results of in vivo experiments showed that the addition of edaravone into chitosan/hyaluronan resulted in faster healing of skin wounds in ischemic rabbits. As proved also by histological investigation, the incorporation of laponite, C-nanofibers or silver-plated C-nanofibers into the composite membranes had significantly beneficial effects on skin wound healing.

Keywords: Artificial skin; Free-radical scavenging capacity; Proliferation and remodelation phases of skin wounds; Wound dressings

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-022-02553-x

Chemical Papers 77 (4) 1835–1841 (2023)