ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Lysozyme modified cryogels for efficient bacteria removal

Nurhan Günay, Ulviye Kilimci, Gizem Öztürk, Deniz Aktaş Uygun, and Murat Uygun

Köşk Vocational School, Food Processing Department, Aydın Adnan Menderes University, Aydın, Turkey

E-mail: muratuygun@gmail.com

Received: 7 February 2023  Accepted: 31 May 2023

Abstract:

In this presented study, the synthesis of poly(HEMA-EH) cryogel, lysozyme immobilization, and bacteria removal efficiency of the immobilized system were described. For this, poly(HEMA-EH) cryogels were prepared by cryocopolymerization technique at sub-zero temperatures by using monomer HEMA and functional epoxy-bearing monomer of EH. Synthesized cryogel was characterized by FTIR, SEM, and an optical microscope. Maximum lysozyme loading was calculated as 43.56 mg/g cryogel. Kinetic parameters of free and immobilized lysozyme were also determined. Finally, the bactericidal effect of the immobilized lysozyme was studied toward model bacteria Micrococcus lysodeikticus, and efficient bacteria removal was demonstrated by staining.

Keywords: Lysozyme; Cryogel; Bacteria removal; Immobilization

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-023-02901-5

Chemical Papers 77 (10) 5839–5846 (2023)