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A unique application of pentane-2,4-dione as a fluorogenic probe of dipeptidyl peptidase-4 enzyme inhibitor medicament assay in pharmaceutical and biological matrices>

Mohamed A. El Hamd, Bassam Shaaban Mohammed, Wael A. Mahdi, Sultan Alshehri, and Ahmed A. Abu-Hassan

Department of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, Shaqra, Saudi Arabia



Received: 20 May 2023  Accepted: 25 September 2023


Sitagliptin (STG) is assigned pharmacologically as a dipeptidyl peptidase-4 enzyme inhibitor. The aforementioned drug, which is taken by mouth either alone or in conjunction with metformin, is used to manage class 2 diabetes. In the proposed study, the ideal utility of the dihydropyridine derivative was applied for STG assessment in an affordable, sensitive, and economical way. The functional amine primary group in STG enables coupling with pentane-2,4-dione to create a fluorescent molecule with the aid of formaldehyde (7.2% v/v) in the reaction. Spectrofluorimetric excitation and emission processes, respectively, at 421.7 and 478.9 nm were used to follow the developed yield of dihydropyridine fluorophore. The experimental circumstances were thoroughly inspected and controlled appropriately. The calibration plot was produced by plotting values of fluorescence intensities against STG concentrations. However, the regulated linearity was observed at STG concentration intervals from 0.1 to 1.5 µg/mL. The effectiveness of the technique was evidenced by a detailed inspection of the ICH criteria. The technique for monitoring STG in various dosage forms and/or spiking biological fluids was effectively used for research purposes. The presently developed method utility for pentane-2,4-dione is a simple, effective, and quick alternative tool for quality control and clinical study assays of STG.

Keywords: Sitagliptin; Fluorescence; Pentane-2,4-dione; Plasma; Urine

Full paper is available at

DOI: 10.1007/s11696-023-03112-8


Chemical Papers 78 (1) 577–586 (2024)

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