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Anticancer potential of Cu4O3 NPs against human ovarian teratocarcinoma: an in-vitro validation

Nasimudeen R. Jabir, Aisha Mahboob, Mohd Suhail, Mohd Shahnawaz Khan, Mohammed Arshad, and Shams Tabrez

Department of Biochemistry, Centre for Research and Development, PRIST University, Vallam, Thanjavur, India



Received: 6 September 2023  Accepted: 3 December 2023


The green approach of synthesizing nanoparticles is an efficient, cost-effective, environmentally friendly, and rapid technique in which plant sources act as capping/stabilizing and reducing agents. The current study aimed to investigate the anticancer potential of biosynthesized Cu4O3 NPs against the human ovarian teratocarcinoma cell line (PA-1). Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and selected area electron diffraction (SAED) were used to predict the size and morphology of the biosynthesized nanoparticles. The SEM image revealed a 200 nm size of Cu4O3 NPs; however, TEM data showed the spherical shape of the Cu4O3 NPs in the range of ≤ 100 nm. A range of biological evaluations, such as cytotoxicity assay, morphological alteration, induction of apoptosis by three different staining techniques (AO/EB dual staining, DAPI, and PI staining), ROS production, and alteration in mitochondrial membrane potential were performed. We observed a dose-dependent cytotoxicity of Cu4O3 NPs in the PA-1 cell line with an IC50 dose of 9.5 µg/ml. Furthermore, Cu4O3 NP treatment induced apoptosis, which was confirmed by all three adopted staining techniques while inducing ROS production and modulating mitochondrial membrane potential. Our findings highlight the anticancer potential of Cu4O3 NPs against the studied cell line, which needs to be further explored because of its cost-effectiveness and eco-friendly nature.

Keywords: Anticancer; Apoptosis; Biogenic Cu4O3 NPs; Ovarian cancer

Full paper is available at

DOI: 10.1007/s11696-023-03272-7


Chemical Papers 78 (5) 2811–2821 (2024)

Monday, May 27, 2024

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