ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Cystine coupled tyrosine modified disposable pencil graphite electrode for the simultaneous analysis of adenine and thymine

Sreelekshmi Premchanth Jyothi, Devika Vinod, Devu Chandran, Sandhya Sadanandan, and Rejithamol Rajamani

Department of Chemistry, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, India

E-mail: sandhya129@gmail.com

Received: 6 July 2024  Accepted: 21 November 2024

Abstract:

In the quest for better diagnostic tools for nucleic acid detection, the present study proposes an innovative approach using a disposable pencil graphite electrode modified with a unique combination of cystine-coupled tyrosine peptide chains. The devised electrochemical sensor has exceptional sensitivity and selectivity for determining adenine and thymine, two pivotal nucleobases. The disposable nature of the electrode increases its practicality and ease of use, while the synergistic interaction between the amino acids cystine and tyrosine contributes to better electrocatalytic activity, allowing precise and simultaneous analysis capabilities. The pencil graphite electrode was modified with cystine-coupled tyrosine using the electropolymerisation method. The potential selectivity of electropolymerization allows for the tailored development of polymers with specific functional group characteristics, offering a promising avenue for sensor development. The fabricated sensor exhibited cost-effectiveness, stability, selectivity, and versatility in detecting adenine and thymine.

Graphical abstract

Keywords: Cystine coupled tyrosine; Adenine; Thymine; Electropolymerisation; Pencil graphite electrode; Artificial blood serum; Artificial urine

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-024-03824-5

Chemical Papers 79 (2) 897–913 (2025)