ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Silver nanoparticles as an effective tool for eradication and control of biofilm-forming Staphylococcus epidermidis isolates

Mohamed M. Gharieb, Amal F. Makled, Sahar M. Ali, Mostafa M. Amin, Kamel R. Shoueir, and Asmaa S. Sleem

Botany and Microbiology Department, Faculty of Science, Menoufia University, Menoufia, Egypt

E-mail: msherwed23@science.menofia.edu.eg

Received: 5 June 2024  Accepted: 9 December 2024

Abstract:

This study examined biofilm-producing Staphylococcus epidermidis isolates from Menoufia University Hospitals, Egypt, and the antibiofilm effects of AgNPs, chlorhexidine, and iodine. About 162 staphylococcal isolates from clinical samples were phenotypically identified as S. epidermidis using Vitek 2, with 71 (43.82%) verified. Tissue culture plate detection and conventional PCR genotyping for ica genes revealed biofilm-producing phenotypes. S. epidermidis isolates developed biofilms (94.36%, 67/71) when subjected to various antiseptic concentrations and durations, including chlorhexidine digluconate (0.025%, 0.035%, and 0.05%), povidone-iodine (3.5%, 7.5%, and 10%), and AgNPs (100, 75, 50, and 25 μg/ml). All biofilms were suppressed by chlorhexidine at 0.05% concentration and varying exposure durations. All povidone-iodine concentrations worked at 10 min; however, 3.5% was ineffective at 5 min. Only 10% concentration prevented biofilm growth at 1 min. AgNPs' antibiofilm impact is concentration-dependent, with the most effective concentration at 100 μg/ml (79.42%), followed by 75 μg/ml (74.91%), 50 μg/ml (70.71%), and 25 μg/ml (62.83%). Chlorhexidine was efficacious in vitro at a therapeutically available concentration of 0.05% and a short exposure duration of 1 min, but povidone-iodine required greater concentrations and longer exposure times. The study found that AgNPs have varying antibiofilm effects, with the most robust inhibition at 100 μg/ml concentration.

Keywords: Staphylococcus epidermidis; Chlorhexidine; Povidone-iodine; AgNPs; Biofilm

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-024-03858-9

Chemical Papers 79 (3) 1355–1366 (2025)