ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Phytochemical characterization and bioactive potential of Daphne jejudoensis

Chang-Dae Lee, Hyeonjun Yu, Neil Patrick Uy, Hwanhee Yu, Chang-Ha Lee, Hoon Kim, and Sanghyun Lee

Department of Plant Science and Technology, Chung-Ang University, Anseong, Republic of Korea

E-mail: saphead1106@hanmail.net

Received: 9 November 2024  Accepted: 12 January 2025

Abstract:

The bioactive potential of Jeju-Baek-Seohyang (JBS), scientifically known as Daphne jejudoensis M. Kim, was extensively investigated. The antioxidant activity of JBS was relatively lower compared to ascorbic acid in the DPPH and ABTS⁺ assays. Despite this, JBS exhibited significant anti-inflammatory effects in lipopolysaccharide-stimulated RAW 264.7 cells showing an IC₅₀ value of 187.0 µg mL⁻¹, which was relatively lower than that of the Centella asiatica sample (77.0 µg mL⁻¹). Furthermore, the JBS sample evaluated in the cytotoxicity assay showed more than 100% RAW 264.7 cell viability even at 250 ppm, a higher concentration than the C. asiatica sample. To characterize the phytochemicals responsible for these bioactivities, LC-ESI/MS and HPLC analyses were employed wherein daphnetin 7-O-glucoside (57.5 mg g⁻¹) and daphnetin (6.5 mg g⁻¹) were identified as the primary flavonoids in the extracts. This study contributes to the evaluation of the potential of JBS, a recently reclassified species that remains understudied, as a valuable medicinal and industrial substance. Further studies are proposed to fully characterize the medicinal properties of this plant.

Keywords: Jeju-Baek-Seohyang; Daphne jejudoensis; Antioxidant activity; Nitric oxide inhibitory activity; LC-ESI/MS; HPLC

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-025-03898-9

Chemical Papers 79 (3) 1915–1925 (2025)