ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Isolation and Characterization of Glycoproteins from the Yeast Cryptococcus laurentii var. laurentii. II. Extracellular Glycoproteins

P. Bartek, N. Kolárová, and P. Capek

Institute of Chemistry у Slovak Academy of Sciences, SK-842 38 Bratislav

E-mail: chemkola@savba.sk

Abstract: The extracellular polysaccharide mixture of Cryptococcus laurentii var. laurentii was fractionated into three chemically distinct heteroglycans by differential precipitation. The compositional analysis of glucuronoxylomannan (GXM) revealed D-mannose (48.8 %), D-xylose (43.8 %), and D-glucuronic acid (7.4 %). It contained 2.3 % protein, 2.1 % phosphorus, and had relative molecular mass 2.4 x 10⁶. The glucomannan (GM) contained 6.5 % protein, 1.0 % phosphorus, and consisted of D-mannose (94.2 %) and D-glucose (5.2 %). The relative molecular mass was determined to be 4.5 x 10⁴. The galactoglucoxylomannan (GalGXM) was composed Of D-mannose (70.1 %), D-galactose (18.0 %), D-glucose (5.7 %), and D-xylose (6.3 %). It contained 6.5 % protein, 0.7 % phosphorus, and had relative molecular mass 1.9 x 10⁴. The results of structural analyses of the extracellular glycoproteins showed 1,6-linked α-D-mannopyranan backbone of GM and GalGXM, and 1,3-linked backbone of GXM.

Full paper in Portable Document Format: 554a261.pdf

Chemical Papers 55 (4) 261–268 (2001)