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High-Performance Liquid Chromatography Analysis of Thiazolidine-4-carboxylic Acids in Human Serum Samples

J. Y. Al-Ja'afreh, Š. Hatrík, and E. Havránek

Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, SK-832 32 Bratislava

 

Abstract: In the presented paper we studied analytical characteristics of three thiazolidine-4-carboxylic acids. These compounds obtained by condensation of aldehydes with L-cysteine are interesting from the biological point of view. The proposed method involves a solid phase extraction (SPE) of thiazolidinic acids and their precursor aldehydes from human serum using C18 SPE columns. Separation of the six analytes was achieved within 20 min using a reversed-phase HPLC SEPARON SGX C18 column protected by precolumn, with mobile phase of methanol-water (φr = 55 : 45) containing 5 cm3 of acetic acid per 1 dm3 of mobile phase (apparent pH 5.5). The recoveries of all studied compounds from the human serum were in the range of 86-89 %, the detection limits were 30 ng cm-3 of serum.

Full paper in Portable Document Format: 532a89.pdf

 

Chemical Papers 53 (2) 89–92 (1999)

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