ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

High-Performance Liquid Chromatography Analysis of Thiazolidine-4-carboxylic Acids in Human Serum Samples

J. Y. Al-Ja'afreh, Š. Hatrík, and E. Havránek

Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, SK-832 32 Bratislava

Abstract: In the presented paper we studied analytical characteristics of three thiazolidine-4-carboxylic acids. These compounds obtained by condensation of aldehydes with L-cysteine are interesting from the biological point of view. The proposed method involves a solid phase extraction (SPE) of thiazolidinic acids and their precursor aldehydes from human serum using C₁₈ SPE columns. Separation of the six analytes was achieved within 20 min using a reversed-phase HPLC SEPARON SGX C₁₈ column protected by precolumn, with mobile phase of methanol-water (φ_r = 55 : 45) containing 5 cm³ of acetic acid per 1 dm³ of mobile phase (apparent pH 5.5). The recoveries of all studied compounds from the human serum were in the range of 86-89 %, the detection limits were 30 ng cm^-3 of serum.

Full paper in Portable Document Format: 532a89.pdf

Chemical Papers 53 (2) 89–92 (1999)