ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Determination of Nitric Oxide in Biological Samples Using N, N, N', N'-Tetramethyl-p-phenylenediamine by UV VIS Spectrophotometry

Š. Mesároš and S. Grunfeld

Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak Technical University, SK-812 31 Bratislava

Abstract: A highly sensitive, simple, fast, and specific procedure for the determination of nitric oxide and nitrite, its decay product in biological system is described. The organic dye agent N, N, N', N'-tetramethyl-p-phenylenediamine was used with nitric oxide and nitrite to form a brightly coloured species which can be detected by UV VIS spectrophotometry. The redox dye formed (Wurster's Blue) has three absorption bands in the ultraviolet and visible region at the wavelengths 328 nm, 564 nm, and 612 nm. All three bands can be used for quantitative determination of nitric oxide and nitrite. A detection limit of 15 nmol dm^-3 was achieved for nitric oxide. The method was applied to the measurement of nitric oxide release from macrophage and endothelial cells. The experimental results are compared with those obtained using the Griess method and by electrochemical method.

Full paper in Portable Document Format: 526a741.pdf

Chemical Papers 52 (6) 741–746 (1998)