ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Determination of lead in microvolumes of blood after decomposition of the organic matrix

D. Kalavská

Department of Biology and Ecology of Man, Faculty of Natural Sciences, Comenius University, CS-842 15 Bratislava

Abstract: Modifications of decomposition of the organic matrix of blood have been studied with purpose to determine Pb in micro-bleedings (in volumes of 150 to 300 mm³). To decompose organic compounds combination of wet and dry decomposition with addition of concentrated H₂S0₄ (100 to 200 mm³) has been utilized at the temperature program of 150 °C for 2 h, 300 °C for 2 h, and 550 °C for 2h. The determination of lead itself was carried out using anodic stripping analysis on a mercury working electrode after dissolution of ash of the sample in the mixture (100 mm³) of HN0₃ and HCl (φ_r=1 l) and dilution with water (2 cm³). Concentrations of Pb²⁺ were calculated by linear regression using five additions of the standard Pb²⁺ solution.

Full paper in Portable Document Format: 433a371.pdf

Chemical Papers 43 (3) 371–376 (1989)