ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

An Enzymatic Sensor for the Analysis of Glycerol in Beverages

I. Lapenaite, B. Kurtinaitiene, L. Marcinkeviciene, I. Bachmatova, V. Laurinavicius, and A. Ramanavicius

Laboratory of Bioanalysis, Institute of Biochemistry, 2600 Vilnius, Lithuania

E-mail: arman@bchi.lt

Received: 11 April 2001

Abstract: A biosensor for the determination of glycerol based on bacterial membranes was developed. Bacterial membranes containing pyrroloquinoline quinone (PQQ) dependent glycerol dehydrogenase (GlycDH) were isolated from Gluconobacter sp. 33 and were immobilized on the carbon rod electrode. Phenazine methosulfate (PMS) was applied as redox mediator. The response of the biosensor is not sensitive to the oxygen concentration in the bulk. Maximal selectivity of the biosensor was observed at pH 8. The calibration graph of the constructed biosensor was linear up to 8-10 mmol dm⁻³ glycerol and possessed the correlation coecient of 0.997. The selectivity of this biosensor to various compounds mostly found in the beverages was investigated. The concentration of glycerol in beverages was determined after biochemical elimination of glucose from the sample.

Full paper in Portable Document Format: 556a345.pdf

Chemical Papers 55 (6) 345–349 (2001)