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Immobilization of thiol peptides and proteins on poly(thioglycoloyloxyethyl methacrylate) gel

D. Podhradský and P. Kristian

Department of Organic Chemistry and Biochemistry, Faculty of Natural Sciences, P. J. Šafárik University, 041 67 Košíce

 

Abstract:  PoIy(thioglycoloyloxyethyl methacrylate) gel has been prepared. The reactivity of its SH groups was checked with [14C]phenyl isothiocyanate. Immobilization of thiol peptides and proteins to a carrier can be performed after its activation with 6,6'-dithiodinicotinic acid. The attached compounds can be released with 50 mmol dm-3 dithiothreitol or can be hydrolyzed with enzymes or acids without decomposition of the carrier.

Full paper in Portable Document Format: 354a567.pdf

 

Chemical Papers 35 (4) 567–573 (1981)

Thursday, September 19, 2019

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