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Isolation and determination of maltooligosaccharides

V. Bílik, J. Zemek, K. Babor, R. Sandtnerová, and J. Kozák

Institute of Chemistry, Slovak Academy of Sciences, 809 33 Bratislava

 

Abstract: Maltooligosaccharides (up to maltooctaose) were converted to the corresponding 4-nitrophenylhydrazones and sepd. by paper chromatog. A method of detn. of maltooligosaccharides in mixts. was elaborated which includes elution of the sepd. 4-nitrophenylhydrazones from the paper and their spectrophotometric measurement. The anal. procedure was employed for assays of amylolytic activities in urine of healthy persons and patients with acute pancreatitis. Isolation of [U-14C]maltooligosaccharides from a [U-14C]starch hydrolyzate via 4-nitrophenylhydrazones is also described.

Full paper in Portable Document Format: 344a524.pdf

 

Chemical Papers 34 (4) 524–529 (1980)

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