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Spectrofluorimetric determination of uric acid based on its activation of catalytic oxidation of pyronine Y

Suling Feng and Xueping Liu

College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, 453007, China



Received: 11 April 2007  Revised: 15 July 2007  Accepted: 17 July 2007

Abstract: A novel kinetic spectrofluorimetric method for the determination of uric acid based on the activation effect of uric acid on the Cu(II) ion catalyzed oxidation of pyronine Y by hydrogen peroxide was developed. The influence of different buffer solutions was tested and the Britton-Robinson buffer solution with pH 2.2 was found to be the optimum. The detection limit and the linear range for uric acid are 0.09 μg mL−1 and 0.3–3.0 μg mL−1, respectively. The RSD for eleven determinations of 1.6 μg mL−1 uric acid was 1.6 %. Satisfactory results were obtained when using this method of uric acid determination in human urine.

Keywords: kinetic spectrofluorimetric method - activation - uric acid - pyronine Y

Full paper is available at

DOI: 10.2478/s11696-008-0029-8


Chemical Papers 62 (3) 318–322 (2008)

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