ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Separation of quinone 2,4-dinitrophenylhydrazones by thin-layer chromatography

B. Rittich and M. Šimek

Research Institute of Animal Nutrition, 691 23 Pohořelice

Abstract: Chromatographic methods by which benzoquinones and naphthoquinones can be identified after their conversion to the corresponding 2,4-dinitrophenylhydrazones are described. Compounds difficult to separate without derivatization can be separated by adsorption chromatography on Silufol^® using systems based on carbon tetrachloride and cyclohexane as the nonpolar, and ethanol and isopropanol as the polar components. Naphthoquinone azo dyes can also be satisfactorily separated on silica gel or alumina impregnated with ß,ß'-oxydipropionitrile when benzene—petrolether mixture (1 1) is used as the mobile phase. The formation of various isomeric azo dyes is also discussed.

Full paper in Portable Document Format: 302a208.pdf

Chemical Papers 30 (2) 208–212 (1976)