ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Optimization of Selenium Determination in Vegetables, Fruits, and Dairy Products by Flow Injection Hydride Generation Atomic Absorption Spectrometry
Food Research Institute, SK-824 75 Bratislava
Received: 16 November 2002
Abstract: A flow injection hydride generation atomic absorption spectrometric (FI-HG-AAS) method was developed for determination of selenium in vegetables fruits, and dairy products after microwave digestion of samples. The experimental conditions for FIAS 400 (concentration of reducing agent and carrier acid, time of reduction, flow rate of argon carrier gas) were optimized. The linearity range under optimized conditions was 0.20-25.0 µg dm-3.
The detection limit of the proposed method was 0.06 µg kg-1 and limit of quantization was 0.20 µg kg-1. The procedure was validated by the method of standard additions (5.0 µg dm-3 and 10.0 µg dm-3 in vegetables, fruits, and dairy products) and the recoveries were from 88 % to 104 %. The accuracy was evaluated using BCR 150 skin milk reference material (found: 0. 128 mg kg-1, sx = 0.004 mg kg-1 certified: 0. 132 mg kg-1, sx = 0.010 mg kg-1). The combined standard uncertainty of selenium was 8.4 %. The method was developed and used for determination of selenium in vegetables fruits and dairy products consumed in Slovakia.
Full paper in Portable Document Format: 573a155.pdf
Chemical Papers 57 (3) 155–157 (2003)