ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Quantitative determination of 3,4-benzopyrene in mixtures, by fluorescence spectrography at -197°

J. Jäger and O. Lugrová

Institut für Hygiene, Praha

Abstract: The quant. detn. of 3,4-benzopyrene (I) by fluorescence spectroscopy at -197°, by adding a standard soln. of I to the analyzed sample, was described. A C₆H₆ ext. of the sample is analyzed by routine thin-layer chromatography on Al₂O3. The fraction so prepd. contains, in addn. to I, other polycyclic aromatic hydrocarbons which cannot be sepd. It is evapd. and dissolved in chromatographically purified n-heptane. It is divided into 3 equal parts, to which is added 0.03, 0.06, and 0.10 γ of a standard soln. of I, so that there is ≤0.15 γ in 1 ml. The prepd. solns. are analyzed with fluorescence spectroscopy. A diagram of the exptl. device with the glass Dewar vessel and the cuvet with the sample are illustrated. Synthetic I mixts. contg. anthanthrene, perylene, 1,2-benzopyrene, 1,12-benzoperylene, 11,12-benzofluoranthrene, and exhausts from automobiles were analyzed. The results were in agreement with the results of the classical spectrophotometric method. The relative error was <8%.

Full paper in Portable Document Format: 1910a774.pdf (in Czech)

Chemical Papers 19 (10) 774–782 (1965)