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Simultaneous spectrophotometric determination of minoxidil and tretinoin by the H-point standard addition method and partial least squares

Maryam Bordbar, Ali Yeganeh-Faal, Jahanbakhsh Ghasemi, Mohammad Mahdi Ahari-Mostafavi, Nahid Sarlak, and Mohammad Taghi Baharifard

Department of Chemistry, Islamic Azad University, Qom-Branch, Qom, Iran



Received: 16 May 2008  Revised: 16 August 2008  Accepted: 4 September 2008

Abstract: A simple, sensitive and selective spectrophotometric method for simultaneous determination of tretinoin and minoxidil using partial least square (PLS) calibration and H-point standard addition method (HPSAM) is described. The results of the H-point standard addition method show that minoxidil and tretinoin can be determined simultaneously with the concentration ratio of tretinoin to minoxidil varying from 2: 1 to 1: 33 in mixed samples. A partial least squares multivariate calibration method for the analysis of binary mixtures of tretinoin and minoxidil was also developed. The total relative standard error for applying the PLS method to eleven synthetic samples in the concentration range of 0–10 μg mL−1 tretinoin and 0–32 μg mL−1 minoxidil was 2.59 %. Both proposed methods (PLS and HPSAM) were also successfully applied in the determination of tretinoin and minoxidil in several synthetic pharmaceutical solutions.

Keywords: minoxidil - tretinoin - partial least squares - H-point standard addition method - simultaneous determination

Full paper is available at

DOI: 10.2478/s11696-009-0003-0


Chemical Papers 63 (3) 336–344 (2009)

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