ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

A spectrofluorimetric method for the determination of acitretin in pharmaceuticals

Suling Feng, Yi Zhang, and Jing Fan

College of Chemistry and Environmental Science, Henan Normal University, 453007 Xinxiang, China

E-mail: slfeng@henannu.edu.cn

Received: 8 October 2008  Revised: 12 December 2008  Accepted: 22 December 2008

Abstract: A simple, rapid, and highly sensitive spectrofluorimetric method for the determination of acitretin was developed based on the strong green fluorescence of acitretin. Influence of organic solvents on the fluorescence spectra of acitretin was studied. Effects of pH, standing time, and foreign ions on the determination of acitretin were also examined. Under the optimum conditions, linear relationship between the relative fluorescence intensity and the concentration of acitretin in the range of 30.0–1100 ng mL⁻¹ was obtained. Detection limit of this method is 9.56 ng mL⁻¹ for acitretin. Relative standard deviation for the determination of 480 ng mL⁻¹ of acitretin was 1.70 %. This method was used for the determination of acitretin in pharmaceuticals and the results were compared with those obtained by the HPLC method.

Keywords: acitretin - spectrofluorimetry - quantitative analysis

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-009-0041-7

Chemical Papers 63 (4) 484–488 (2009)