ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Determination of metoprolol tartrate by capillary isotachophoresis
Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin str., 87-100 Toruń, Poland
Received: 17 April 2009 Revised: 12 October 2009 Accepted: 19 October 2009
Abstract: This paper describes two isotachophoretic methods of metoprolol tartrate (MT) determination in pure and dosage forms. The first method was used for direct analysis where the following electrolyte system was applied: 10 mmol dm−3 3-morpholino-2-hydroxypropanesulfonic acid, 10 mmol dm−3 NaCl, 2 % hydroxyethylocelulose as leading (LE) and 10 mmol dm−3 glycyl-glycine as terminating (TE) electrolytes. The second method was used for indirect analysis of MT as tartrate ions. In this case, the leading electrolyte consisted of 10 mmol dm−3 HCl, β-alanine (BALA), pH 4-5, and the terminating one of 5 mmol dm−3 glutamic acid, 10 mmol dm−3 β-alanine. Calibration curves were calculated as follows: for system A: y = (0.52 ± 0.05)x − (0.9 ± 0.2) (LOD = 13.0 mg dm−3, LOQ = 31.7 mg dm−3); and for system B: y = (0.240 +- 0.001)x + (0.18 ± 0.06) (LOD = 1.8 mg dm−3, LOQ = 4.4 mg dm−3). The isotachophoretic method was compared with the pharmacopoeial one by statistical tests.
Keywords: capillary isotachophoresis - metoprolol tartrate - Metocard - Metohexal - Betaloc
Full paper is available at www.springerlink.com.
Chemical Papers 64 (3) 294–300 (2010)