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Effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis in Penicillium janthinellum MTCC 10889

Aditi Kundu and Rina Rani Ray

Microbiology Research Laboratory, Post Graduate Department of Zoology, Molecular Biology and Genetics, Presidency University, 86/1, College Street, Kolkata, 700 073, India

 

E-mail: raypumicro@gmail.com

Abstract: Amongst various carbon sources, xylan was found to be the sole inducer of endoxylanase production by Penicillium janthinellum MTCC 10889 in submerged cultivation. Endoxylanase synthesis by a xylan induced culture was initially repressed after a simultaneous addition of xylose, probably by the inducer exclusion mechanism, but it was resumed and achieved its highest level at a much later stage of growth (at 120 h). Xylose added after 30 h of growth cannot exert its full repressive effect. Although glucose was proved to be a more potent repressor than xylose, supplementation of salicin, an alcoholic β-glycoside containing d-glucose, with pure xylan resulted in an about 3.22 fold increase in the enzyme synthesis at 72 h followed by constant high production of the enzyme at least until the 144th h of growth. Inducing capacity of salicin in a xylan induced culture was significantly reduced when it was added after 30 h of growth. Addition of salicin and xylan help to partially overcome the repressive effect of xylose and glucose. Failure of salicin in recovering the endoxylanase synthesis in actinomycin D and cyclohexamide inhibited the xylan induced culture indicating that salicin cannot initiate the de novo synthesis of the enzyme.

Keywords: endoxylanase – induction – Penicillium janthinellum – salicin – repression

Full paper is available at www.springerlink.com.

DOI: 10.2478/s11696-013-0478-6

 

Chemical Papers 68 (4) 451–456 (2014)

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