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Application of polypyrrole nanowires for the development of a tyrosinase biosensor

Jolanta Kochana, Katarzyna Hnida, Grzegorz Sulka, Paweł Knihnicki, Joanna Kozak, and Agnieszka Gilowska

Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland

 

E-mail: kochana@chemia.uj.edu.pl

Abstract: Polypyrrole nanowires (PPyNWs) were fabricated and examined as a structural component of amperometric biosensor matrix. An enzyme, tyrosinase (TYR), was immobilized onto PPyNWs using glutaraldehyde (GA). Matrix composite morphology was investigated using scanning electron microscopy. Electrochemical behavior of the prepared PPyNWs/GA/TYR biosensor towards catechol was studied and the assessment of its analytical characteristics was carried out taking into account linear range, sensitivity, repeatability, reproducibility and operational stability.

Keywords: polypyrrole nanowires – conductive polymers – biosensor – tyrosinase – immobilization

Full paper is available at www.springerlink.com.

DOI: 10.1515/chempap-2015-0114

 

Chemical Papers 69 (8) 1130–1135 (2015)

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