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Fabrication of amperometric cholesterol biosensor based on SnO2 nanoparticles and Nafion-modified carbon paste electrode

Gözde Aydoğdu Tığ, Derya Koyuncu Zeybek, and Şule Pekyardımcı

aDepartment of Chemistry, Faculty of Science, Ankara University, Ankara 06100, Turkey



Abstract: This study reports the fabrication of an amperometric cholesterol biosensor based on cholesterol oxidase (ChOx), SnO2NPs and Nafion-modified carbon paste enzyme electrodes (CPE/SnO2NPs-ChOx/Naf). The electrochemical characterisations of BCPE and CPE/SnO2NPs were performed using CV and EIS. The determination of cholesterol was carried out by electrochemical oxidation of H2O2 at 0.6 V vs. Ag/AgCl. The CPE/SnO2NPs-ChOx/Naf presented a linear range from 0.20 μmol L−1 to 4.95 μmol L−1 with a low limit of detection (0.04 μmol L−1 ). In addition, the optimal values for pH and temperature were found to be 7.5 and 35°C, respectively. The CPE/SnO2NPs-ChOx/Naf was used for the determination of cholesterol in serum samples and good results were obtained

Keywords: cholesterol biosensor – carbon paste electrode – amperometry – SnO2 nanoparticles

Full paper is available at

DOI: 10.1515/chempap-2016-0005


Chemical Papers 70 (6) 695–705 (2016)

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