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Determination of non-polar heterocyclic aromatic amines in roasted coffee by SPE-HPLC-FLD

Deimantė Karpavičiūtė, Michael Murkovic, Rimantė Vinauskienė, and Rimantas Venskutonis

Department of Food Science and Technology, Kaunas University of Technology, Kaunas, Lithuania



Abstract: The non-polar heterocyclic aromatic amines (HAAs) are formed as pyrolysis products of amino acids at higher temperatures. Green coffee beans were roasted at 240 °C from 5 to 14.5 min (four levels). Solid-phase extraction coupled to high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was used for identification and quantification of five HAAs (Glu-P 2, Glu-P 1, Phe-P 1, AαC, and MeAαC) in roasted coffee. The excitation and emission wavelength program was optimized for non-polar HAAs. The analytes were eluted with eluent A (76% water, 14% acetonitrile, 8% methanol, and 2% acetic acid; pH = 5) and B (acetonitrile) in gradient mode. The limits of detection for non-polar HAAs were in the range of 0.21–0.51 ng/g, the limits of quantification from 0.38 to 0.93 ng/g with a recovery of 40–56%. From the tested HAAs, only AαC was present in roasted coffee; Glu-P 2, Glu-P 1, Phe-P 1, and MeAαC were not found in any coffee sample.

Keywords: Non-polar heterocyclic aromatic amines; Roasted coffee; SPE-HPLC-FLD

Full paper is available at

DOI: 10.1007/s11696-016-0039-x


Chemical Papers 71 (1) 67–70 (2017)

Thursday, August 18, 2022

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