ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Bioconversion, purification and characterization of tylvalosin

Jia-Xin Wu, Chuan-Liang Zhang, Jin-Lei Xu, Guo-Dong Zhang, Jian Hao, Miao Zhang, Min Song, Peng Qi, Gang Zhang, and Jing-Le Liang

China Animal Husbandry Industry Co., Ltd, Beijing, China

E-mail: icebergwujiaxin@163.com

Abstract: Tylvalosin is a new macrolide antibiotic for veterinary use. The study reported in this paper dealt with the systematic bioconversion, purification and characterization of tylvalosin. In this work, tylvalosin was synthesized by a bioconversion using Streptomyces thermotolerans with tylosin as the substrate, in which the final yield was 8148 U mL⁻¹. Subsequently, the crude tylvalosin was obtained from an acid and alkali recrystallization of the fermentation broth. High-purity tylvalosin was subsequently obtained from silica gel column chromatography separation. In the advanced separation, silica gel was the separation medium and a mixture of ethyl acetate and petroleum ether (contained 0.1% triethylamine) was used as the eluent. The activity of the pure tylvalosin was 996 U mg⁻¹ as determined by microbiological assay and the purity of the product was 96.0% as determined by high-performance liquid chromatography. The tylvalosin product was qualitative analysed using ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry. The structure of the tylvalosin product was characterized using infrared spectroscopy and nuclear magnetic resonance analysis. The crystal structure of the tylvalosin was determined using by X-ray single crystal diffraction. In summary, this study established a complete method for the biotransformation, separation, purification and characterization of tylvalosin.

Keywords: Tylvalosin; Bioconversion; Purification; Characterization

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-017-0222-8

Chemical Papers 71 (11) 2283–2291 (2017)