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ISSN electronic edition: 1336-9075
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Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids

Jing Ke, Xiao-Yun Duan, Xiao-Feng Liu, and Yu-Ming Dong

Lanzhou University, Lanzhou, China



Abstract: In this work, an isocratic micellar chromatographic method for separation of 17 free amino acids was first established. Mixed anionic and non-ionic surfactants were used as mobile phase additives. Besides, a chromatographic environment was simulated and the possible mechanism was discussed. Pre-column derivatization with 9-fluorenylmethyl chloroformate was used before chromatographic analysis. The optimized chromatographic conditions were the mobile phase A (0.075 M sodium dodecyl sulfate solutions and 0.010 M polyoxyethylene lauryl ether containing 20 mM ammonium acetate at pH 3.5) and B (100% acetonitrile) (85:15, v/v) running at 1.2 mL/min by a Venusil XBP C18 column (5 µm, 250 × 4.6 mm) at 35.0 °C. Compared with the conventional reversed phase liquid chromatography, the consumption of organic solvent in the method is lower which reduces the analysis cost and being environment-friendly. In addition, complex gradient elution is not required. This provides an alternative way for the separation of amino acids.

Keywords: Amino acids ; Micellar liquid chromatography ; Mixed surfactants ; Polyoxyethylene lauryl ether ; Sodium dodecyl sulfate 

Full paper is available at

DOI: 10.1007/s11696-019-00791-0


Chemical Papers 73 (10) 2417–2426 (2019)

Tuesday, May 21, 2024

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