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ISSN electronic edition: 1336-9075
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Development of a simple and rapid method for determination of trans, trans-Muconic Acid in human urine using PDLLME preconcentration and HPLC–UV detection

Masoud Rismanchian, Karim Ebrahim, and Zahra Ordudari

Isfahan University of Medical Sciences, Isfahan, Iran



Abstract: Benzene is a known human carcinogen and monitoring its exposure is very important. trans, trans-Muconic Acid (tt-MA) is an open-ring metabolite product proposed by related regulatory bodies as the best biomarker of benzene exposure. In this study, a new, simple and rapid method was developed for determination of tt-MA in a urine sample using partitioned dispersive liquid–liquid microextraction technique coupled with high-performance liquid chromatography–UV detection. Under the optimal condition, 2000 µL of tetrahydrofuran and 200 µL chloroform were used as disperser and extraction solvent, respectively, for 5 mL sample containing the target analyte. The method was linear in the range of 0.001–10 mg L−1. Method LOD, LOQ, relative standard deviation (RSD) for 3 days and under three concentrations and relative recovery were obtained 0.11 μg L−1, 0.83 µg L−1, 2.02–3.96% and 86–102%, respectively. Therefore, the proposed method with high recovery and easy application is recommended to be used to detect tt-MA in human urine samples.

Keywords: Biological monitoring ; HPLC ; Partitioned dispersive liquid–liquid microextraction (PDLLME) ; trans,trans-Muconic Acid (tt-MA) ; Urine 

Full paper is available at

DOI: 10.1007/s11696-019-00800-2


Chemical Papers 73 (10) 2485–2492 (2019)

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