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Enzymatic saccharification of cellulose in aqueous-ionic liquid 1-ethyl-3-methylimidazolium dimethylphosphate-DMSO media

Yu C. He, Cui L. Ma, Dong Q. Xia, Liang Ding, Liang Z. Li, and Mu Xian

College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou, 213164 China



Abstract: Ionic liquid (IL) 1-ethyl-3-methylimidazolium dimethylphosphate ([Emim]DMP) was chosen as an environment-friendly solvent to enzymatically hydrolyze cellulose in situ. Under optimal reaction condition, 80.2 % of cellulose (10 mg mL−1) were converted to glucose in aqueous-IL-DMSO (φ r = 74: 25: 1) media at 55°C in 18 h. Finally, fermentability of the recovered hydrolyzates was evaluated using Saccharomyces cerevisiae which is able to ferment hydrolyzates efficiently, the ethanol production was 0.44 g g−1 of glucose within 24 h of the process. Such information is vital for the saccharification of more complex cellulose materials and for the fermentation of hydrolyzates into biofuel.

Keywords: ionic liquid – 1-ethyl-3-methylimidazolium dimethylphosphate – cellulose – enzymatic saccharification – DMSO – optimization

Full paper is available at

DOI: 10.2478/s11696-011-0066-6


Chemical Papers 65 (6) 792–797 (2011)

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