ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
A carbazole-based fluorescent probe for ultra-fast detection of ClO− and its application to live cell imaging
Jianbin Chao, Yuexiang Duan, Yongbin Zhang, Fangjun Huo, Caixia Yin, Miao Xu, and Ming Li
Scientific Instrument Center, Shanxi University, Taiyuan, People’s Republic of China
Received: 24 July 2019 Accepted: 11 October 2019
A new fluorescent probe, (E)-3-(9-ethyl-9H-carbazol-6-yl)-1-(2-hydroxy-5-methoxyphenyl)prop-2-en-1-one (Probe 1), was synthesized based on the carbazole fluorophore for detecting ClO−. The fluorescent probe exhibited specificity for ClO− in many analytes and excellent anti-interference ability during detection. In addition, the fluorescence intensity at 525 nm illustrated a good linear relationship with the concentration of ClO− in the range of 53.6–107.2 μM and the limit of detection was 0.30 μM. Furthermore, fluorescence imaging results confirmed that Probe 1 can be applied to monitor ClO− in HepG2 cells, which indicated that Probe 1 has large potential for monitoring ClO− in vivo.
Keywords: Fluorescence intensity; Carbazole; ClO−; Imaging
Full paper is available at www.springerlink.com.
Chemical Papers 74 (4) 1171–1176 (2020)