ISSN print edition: 0366-6352
ISSN electronic edition: 1336-9075
Registr. No.: MK SR 9/7
Advantageous conditions of saccharification of lignocellulosic biomass for biofuels generation via fermentation processes
Karolina Kucharska, Edyta Słupek, Hubert Cieśliński, and Marian Kamiński
Faculty of Chemistry, Department of Process Engineering and Chemical Technology, Gdańsk University of Technology, Gdańsk, Poland
Received: 30 June 2019 Accepted: 11 October 2019
Processing of lignocellulosic biomass includes four major unit operations: pre-treatment, hydrolysis, fermentation and product purification prior to biofuel generation via anaerobic digestion. The microorganisms involved in the fermentation metabolize only simple molecules, i.e., monosugars which can be obtained by carrying out the degradation of complex polymers, the main component of lignocellulosic biomass. The object of this paper was to evaluate the saccharification conditions and identify the process parameters that should be applied to improve the saccharification efficiency of lignocellulosic biomass, defined as the simple sugars concentration, which was considered as a crucial parameter for hydrogen generation via dark fermentation. Drawing global conclusions about the occurring changes in the biomass requires learning about the nature of the biomass structure and composition at different stages of the process. Therefore, techniques for analysis, as FTIR, HPLC and SEM were applied. The experiment was planned employing Box–Behnken design. The advantageous operating conditions and the composition of saccharification enzymatic cocktail were identified and their values occurred similar in the applied border conditions for all tested biomass types. Analysis of the intermediate solid and liquid streams generated during the pre-treatment procedure revealed several structural and compositional changes in the biomass.
Keywords: Lignocellulosic biomass; Saccharification biohydrogen; Fermentation; Process control methods
Full paper is available at www.springerlink.com.
Chemical Papers 74 (4) 1199–1209 (2020)