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Advantageous conditions of saccharification of lignocellulosic biomass for biofuels generation via fermentation processes

Karolina Kucharska, Edyta Słupek, Hubert Cieśliński, and Marian Kamiński

Faculty of Chemistry, Department of Process Engineering and Chemical Technology, Gdańsk University of Technology, Gdańsk, Poland



Received: 30 June 2019  Accepted: 11 October 2019


Processing of lignocellulosic biomass includes four major unit operations: pre-treatment, hydrolysis, fermentation and product purification prior to biofuel generation via anaerobic digestion. The microorganisms involved in the fermentation metabolize only simple molecules, i.e., monosugars which can be obtained by carrying out the degradation of complex polymers, the main component of lignocellulosic biomass. The object of this paper was to evaluate the saccharification conditions and identify the process parameters that should be applied to improve the saccharification efficiency of lignocellulosic biomass, defined as the simple sugars concentration, which was considered as a crucial parameter for hydrogen generation via dark fermentation. Drawing global conclusions about the occurring changes in the biomass requires learning about the nature of the biomass structure and composition at different stages of the process. Therefore, techniques for analysis, as FTIR, HPLC and SEM were applied. The experiment was planned employing Box–Behnken design. The advantageous operating conditions and the composition of saccharification enzymatic cocktail were identified and their values occurred similar in the applied border conditions for all tested biomass types. Analysis of the intermediate solid and liquid streams generated during the pre-treatment procedure revealed several structural and compositional changes in the biomass.

Keywords: Lignocellulosic biomass; Saccharification biohydrogen; Fermentation; Process control methods

Full paper is available at

DOI: 10.1007/s11696-019-00960-1


Chemical Papers 74 (4) 1199–1209 (2020)

Friday, March 05, 2021

SCImago Journal Rank 2019
XXVIII. International Conference on Coordination and Bioinorganic Chemistry
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