ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Determination of lobeline in biological fluids

E. Hradský and K. Barna

University of P. J. Šafárik, Košice

Abstract: Lobeline was detd. by a turbidimetric method based on the pptn. of lobeline with Nessler reagent. The method was applied to urine and blood serum. To 10 ml. of fresh urine add 100 γ HgCl₂ and 1 ml. 2N NaOH, filter, and to the filtrate add 2 ml. 10% HCl and 0.2 ml. Nessler reagent. Measure turbidity after 20 min. at 580 mμ. For blood serum, dialyze 5 ml. with 10 ml. 0.5M NaCl, add 1 ml. 10% HCl and 0.2 ml Nessler reagent to 5 ml. of the dialyzate and measure as with urine. Urinary lobeline concns. of 5-50 γ/ml. (blood 2-20 γ/ml.) were detectable.

Full paper in Portable Document Format: 187a542.pdf (in Slovak)

Chemical Papers 18 (7) 542–546 (1964)