ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Hemoglobin. V. N-terminal amino acids and the number of polypeptide chains in rat hemoglobin

P. Mäsiar

Department of Biochemistry Faculty of Medicine, University of P. J. Šafárik, Košice

Abstract: Rat hemoglobin terminates in 2 α-polypeptidic chains contg. valine and in 2β-polypeptidic chains contg. methionine. From an acid hydrolyzate of dinitrophenylated globin a valylleucyl sequence was isolated which represents the α-polypeptidic chain in hemoglobin. By stepwise degradation with phenylthiohydantoin (I) leucine and glycine were detd. as N-terminal amino acids. Two α-polypeptidic chains are terminated with a valylleucyl sequence and 2β-polypeptidic chains with a methionylglycyl sequence. Quant. detn. of I derivs. with paper chromatography and a modified method of crystn. of hemoglobin are described.

Full paper in Portable Document Format: 1410a717.pdf (in Slovak)

Chemical Papers 14 (10) 717–724 (1960)