ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Screening of anti-inflammatory parts in pine needles and their enrichment by a hydrophilic/hydrophobic diphase monolith

Xuanwen Wei, Jiaqi Guo, Ligai Bai, Hongyuan Yan, and Haiyan Liu

College of Pharmaceutical Sciences, Key Laboratory of Public Health Safety of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, State Key Laboratory of New Pharmaceutical Preparations and Excipients, Hebei University, Baoding, China

E-mail: lhy1610@126.com

Received: 5 January 2026  Accepted: 10 February 2026

Abstract:

Before screening for active ingredients in natural medicines, sample pretreatment is required to improve the purity of the active components. In this paper, a monolith composed of hydrophilic and hydrophobic parts was prepared by a one-step synthesis method. The prepared mixed-mode monolithic material was used as a solid-phase extraction adsorbent for online enrichment and preliminary separation of chemical components in pine needles. When water was used as the enrichment mobile phase, the chemical components (extracted by water or ethanol/water) absorbed on the monolith were eluted using different mobile phase. By this way, fractions with different polarities were obtained. After these fractions were collected and evaporated to dryness, they were redissolved and subjected to activity tests using the CCK-8, ELISA and Griess method. The results showed that all fractions did not have negative impacts on the survival of RAW264.7 cells. Some fractions could reduce the level of inflammatory factors secreted by cells. Among them, Fraction1-2 had a more obvious effect on reducing IL-6, TNF-α and NO levels of cells, which were extracted with water and obtained when water and acetonitrile/water (60/40, V/V) were used as the enrichment and elution mobile phase, respectively. The results of DPPH and ABTS free radical scavenging assays showed that this fraction showed strong in vitro antioxidant activity.

Keywords: Anti-inflammatory parts; Pine needles; One-step preparation; Diphase monolith; Enrichment

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-026-04738-0

Chemical Papers 80 (5) 5709–5716 (2026)