ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Chemical profile and antithrombotic activity of the essential oil of Levisticum officinale W.D.J.Koch (Apiaceae)

Denise Caroline Luiz Soares Basilio, Isabelly Teixeira Espinoça, Paola Mayara Valente Coronel, Rafael Seiji Nakano Ota, Anna Júlia Papa de Araujo, Nadla Soares Cassemiro, Denise Brentan Silva, Janaina de Cássia Orlandi Sardi, Kamylla Fernanda Souza de Souza, Davi Campos La Gatta, Edgar Julian Paredes-Gamero, Everton do Nascimento Alencar, and Eduardo Benedetti Parisotto

Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Bairro Universitário, Campo Grande, Brazil

E-mail: eduardo.parisotto@ufms.br

Received: 22 February 2024  Accepted: 15 June 2024

Abstract:

Cardiovascular diseases generate high costs for public health systems, making it necessary to develop/discover anticoagulant or antiplatelet agents. In this context, the present study investigated the antithrombotic action of the essential oil of Levisticum officinale W.D.J. Koch in an in vitro experimental model. The oil chemical composition was characterized using gas chromatography coupled to mass spectrometry (GC–MS). The trypan blue exclusion assay in platelets (in vitro) was carried out. Systemic toxicity was studied by an in vivo model of Galleria mellonella larvae. Platelet aggregation assay was performed by turbidimetry in an aggregometer device, using the adenosine receptor agonist, ADP, and epinephrine (EPI). Coagulation was determined by prothrombin activity time (PT) and partially activated thromboplastin time (aPTT) in the coagulation system. Platelet activation was assessed by P-selectin expression and reactive oxygen species (ROS) content by flow cytometry and fluorescence, respectively. The oil is composed of monoterpenes, including α-terpinyl acetate, β-phellandrene, β-myrcene, α-terpineol, and (Z)-β-ocimene. The oil did not show toxicity in vitro and in vivo. The oil significantly reduced platelet aggregation against ADP and EPI. EPI-induced aggregation was most potently inhibited. Treatment of platelets with oil showed a decrease in P-selectin expression and ROS production when compared to vehicle. On the other hand, the oil did not interfere in PT and aPTT. The results demonstrated that the essential oil of Levisticum officinale has antiplatelet action but without anticoagulant effects.

Graphical abstract

Keywords: Lovage; Antiaggregant; Platelets; P-selectin; Reactive oxygen species

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-024-03573-5

Chemical Papers 78 (12) 6891–6900 (2024)