ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Identification and quantitation of regenerated casein fibers in combination fabric based on lateral flow immunoassay

Ronghu Feng, Shiwei Zhang, Ying He, Huiling Wu, Jiping Guo, Yingchun Zhou, and Xintian Lai

Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, People’s Republic of China

E-mail: zsw_8506@163.com

Received: 16 April 2024  Accepted: 24 July 2024

Abstract:

Regenerated protein fibers with different biogenetic derivations greatly vary in cost. However, finding a proper method for identifying and quantitating regenerated protein fibers is difficult. This study developed a rapid, inexpensive, convenient, and accurate qualitative immunochromatography method to detect regenerated casein fiber (RCF) in textiles. The RCF was characterized by electrospray ionization/time of flight mass spectrometry. Peptide haptens were designed based on an inert, conserved, and immunogenic sequence of regenerated casein. Monoclonal antibodies were prepared from the haptens, and a colloidal gold immunochromatography assay (GICA) was applied for the detection. A limit of detection of 0.1% RCF in textiles was achieved with a total analysis time of only 15 min using an extraction solution of 7 M urea containing 0.1% dithiothreitol and treatment for 10 min in boiling water. Quantitative assay results were obtained by using a GICA reader with the recovery rates of 110 to 130% and relative standard deviations of ≤ 20%.

Keywords: Regenerated casein fiber; Hapten design; Immunochromatography; Protein fiber identification

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-024-03628-7

Chemical Papers 78 (13) 7643–7650 (2024)