ISSN print edition: 0366-6352 ISSN electronic edition: 1336-9075 Registr. No.: MK SR 9/7 Published monthly

Catalytic performance of lipase-inorganic hybrid nanoflower in the presence of some oils/fat/waste oils and detergents/surfactants

Kezban Yildiz-Dalginli

Department of Chemistry and Chemical Processing Technologies, Kars Vocational HighSchool Kafkas University, Kars, Turkey

E-mail: kezbandalginli@gmail.com

Received: 6 March 2025  Accepted: 1 June 2025

Abstract:

In this study, the conventional hybrid nanoflower synthesis method was used for lipase-inorganic hybrid nanoflowers. Following a comprehensive pH and temperature scanning of the nanoflowers, their activity was investigated in some detergents/surfactants and oils/fats/waste oils to evaluate the activity, stability and compatibility of the biocatalyst. The optimal morphology for L@ihNFs was determined to be 0.05 mg/mL. To obtain the optimum activity, conditions were explored pH values ranging from 6 to 12 and temperatures between 30 and 80 °C. Both the free form and L@ihNF exhibited higher activity in alkaline conditions, with a significant decrease in activity observed at pH 11 and 12. L@ihNF showed superior activity and remarkable compatibility compared to the free enzyme in the presence of various commercial liquid/powder detergents and surfactants. Moreover, it displayed over a twofold increase in activity in some oils/fat/waste oils. The reusability of the synthesized L@ihNF was examined in 10 cycles, and it was observed that it still showed 58% activity at the end of the 8th cycle, which is a good indicator of reusability. L@ihNFs exhibited good storage stability, retaining approximately 50% activity after 20 days compared to the free enzyme.

Keywords: Lipase-inorganic hybrid nanoflowers; Oils/fat/waste oils; Detergent; Surfactants; Catalytic activity

Full paper is available at www.springerlink.com.

DOI: 10.1007/s11696-025-04168-4

Chemical Papers 79 (9) 5997–6010 (2025)