Affordable and reliable fluorescence-based approach for xylometazoline detection in nasal preparations. Comprehensive assessment of whiteness and bluenessDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, Egypt
E-mail: Ahmedabuhassan@azhar.edu.eg Received: 6 September 2025 Accepted: 2 November 2025 Abstract: Xylometazoline is frequently employed to mitigate allergic rhinitis, nasal obstruction, and sinusitis. The current article presents a new development and validates an affordable, reliable, and low-cost fluorescence-based strategy for the xylometazoline assay. The concept of the assay relies on the labeling of xylometazoline with 4-chloro-7-nitrobenzofurazan. The labeled derivative formed under suitable conditions was monitored by adjusting the spectrofluorometer at an emission wavelength of 532.5 nm and setting 472.6 nm as excitation. The calibration graph was drawn by graphing derivative fluorescence against xylometazoline concentration, achieving reliable linearity within a spectrum of 20–600 ng mL⁻1. Rigorous refinement of the procedural affecting variables was performed to get the highest peak of the derivative fluorescence. Validity of the study was asserted by meticulous adherence to ICH rules and the method showed excellent linearity (r = 0.9995) with a limit of quantification of 19.34 ng mL⁻1. The strategy was satisfactorily utilized in the evaluation of xylometazoline in both nasal drops and spray forms. By analyzing these attributes, the insights for the effectiveness and applicability of the methodology were confirmed and provided a framework for comparing its performance against alternative approaches. Keywords: Spectrofluorimetric; Xylometazoline; NBD-Cl; Nasal preparation; Validation Full paper is available at www.springerlink.com. DOI: 10.1007/s11696-025-04487-6
Chemical Papers 80 (2) 1757–1767 (2026) |
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